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Luminescence‐based detection of Erwinia carotovora associated with rotting potato tubers
Author(s) -
McLennan K.,
Glover L.A.,
Killham K.,
Prosser J.I.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00742.x
Subject(s) - erwinia , luminescence , inoculation , biology , oxygen , solanaceae , microbiology and biotechnology , food science , botany , chemistry , bacteria , horticulture , biochemistry , optoelectronics , materials science , genetics , organic chemistry , gene
A luminescence‐based marker system has been used to follow rotting of potato tubers by Erwinia carotovora. The early stages of infection could be detected by visual observation of areas of luminescence developing from the point of inoculation. During later stages luminescence was limited to peripheral regions and to central regions where the structure of the potato was destroyed. Luminometry demonstrated reduction in aerobic activity of infecting cells due to oxygen limitation. The system enables rapid and sensitive detection of active marked populations and distinction between aerobic and fermentative activity.