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Microbial load in poultry feed and detection of aflatoxin B 1 using monoclonal antibody‐based enzyme linked immunosorbent assay
Author(s) -
Banerjee Ameeta,
Shetty H.S.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00733.x
Subject(s) - aflatoxin , monoclonal antibody , biology , veterinary medicine , microbiology and biotechnology , medicine , antibody , immunology
Feed samples collected from different poultry farms and feed mills situated in Andaman and Nicobar islands in India were assessed for microflora and aflatoxin B 1 contamination. The bacterial counts ranged from 1.0 times 10 7 to 8.8 times 10 7 cfu/g of the feeds, while counts of fungi ranged from 1.0 times 10 3 to 8.7 times 10 3 cfu/g. The mycoflora comprised mainly of Aspergillus spp., A. flavus being most dominant. Aflatoxin B 1 was detected by monoclonal antibody‐based enzyme linked immunosorbent assay technique and the content in different feed samples ranged from 5.5 to 90 ng/g.