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Increased disruption resistance of Candida utilis grown from survivors of enzymatic lysis combined with high‐pressure homogenization
Author(s) -
Baldwin C.,
Robinson C. W.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00725.x
Subject(s) - homogenization (climate) , lysis , enzyme , yeast , biology , enzyme assay , microbiology and biotechnology , biochemistry , food science , ecology , biodiversity
The resistance of Candida utilis (ATCC 9226) to disruption as a result of enzymatic pretreatment combined with high‐pressure homogenization was found to increase when the yeast was grown from an inoculum which had previously been subjected to enzymatic pretreatment combined with high‐pressure homogenization. The inoculum thus consisted of a mixture of undisrupted, viable cells and non‐viable cells. The enzyme preparation employed was Zymolyase, which depolymerizes various components of the cell walls of viable yeast. A Microfluidizer was used for the high‐pressure homogenization step. In order to obtain the ‘disruption‐resistant’ cell fraction for use as an inoculum, ‘normal’ C. utilis was enzymatically pretreated, and subsequently homogenized (herein referred to as Microfluidization) using either three or 10 passes through the Microfluidizer at an operating pressure of 95 MPa. Yeast grown from the survivors of the enzyme/3‐pass treatment were found to be somewhat more resistant to disruption by either enzymatic pretreatment alone or to enzymatic pretreatment followed by Microfluidization. Cells grown from enzyme/ 10‐pass treated inocula exhibited the highest resistance to disruption. The ‘disruption‐resistant’ fraction exhibited this characteristic through three serial re‐cultivations. Possible mechanisms for the increased ‘disruption‐resistance’ of this isolated population of C. utilis are presented.