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A highly sensitive enzyme‐linked immunosorbent assay for Clostridium perfringens enterotoxin
Author(s) -
Uemura T.,
Yoshitake S.,
Hu D.,
Kajikawa T.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00714.x
Subject(s) - clostridium perfringens , enterotoxin , microbiology and biotechnology , clostridiaceae , detection limit , enzyme , chemistry , toxin , clostridium , chromatography , biology , bacteria , biochemistry , escherichia coli , genetics , gene
A highly sensitive enzyme‐linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.