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A simple procedure for the preparation of lipopolysaccharide for the production of antisera
Author(s) -
Chart H.,
Rowe B.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00701.x
Subject(s) - rowe , chart , public health , antiserum , library science , microbiology and biotechnology , operations research , biology , medicine , computer science , management , immunology , pathology , antigen , mathematics , statistics , economics
Lipopolysaccharide (LPS) was isolated from a strain of Escherichia coli O157 by two different methods and used to prepare antisera in rabbits. LPS prepared by the proteolytic digestion of whole cells was identical to LPS prepared with hot‐phenol, as shown by SDS‐PAGE and silver staining. Antisera from rabbits receiving these LPS preparations contained high titred antibodies (>10 5 ) of the IgM class to E. coli O157 LPS.