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RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA
Author(s) -
Mazurier S.,
Giessen A. van de,
Heuvelman K.,
Wernars K.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00700.x
Subject(s) - rapd , primer (cosmetics) , biology , dna profiling , serotype , dna , campylobacter , polymerase chain reaction , genetics , campylobacter jejuni , microbiology and biotechnology , bacteria , gene , chemistry , genetic diversity , population , medicine , organic chemistry , environmental health
A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR‐based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10‐mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.