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Suitability of a rapid gas chromatographic method for total mesophilic bacteria and coliform enumeration in hamburgers
Author(s) -
Basem A.,
Gardini F.,
Paparella A.,
Guerzoni Maria Elisabetta
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00699.x
Subject(s) - mesophile , enumeration , chromatography , bacteria , gas chromatography , chemistry , coliform bacteria , environmental science , food science , biology , mathematics , genetics , combinatorics
An instrumental method for the rapid determination of total mesophilic bacteria and total coliforms in hamburgers is described. The two procedures are based on the gas chromatographic determination of metabolic CO 2 in equilibrium in the head space of sealed vials containing suitable media and inoculated with aliquots of the sample under examination. Both procedures proved to be suitable for quality control of meat products, being highly sensitive, reproducible, simple and rapid. Good correlation coefficients were obtained with plate counting for microbial concentrations ranging between 10 1 and 10 8 .