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In vitro effects of the polyamine biosynthesis inhibitor, α‐difluoromethylornithine, on ornithine decarboxylase activities from three plant pathogenic fungi
Author(s) -
Havis N.D.,
Walters D.R.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00696.x
Subject(s) - ornithine decarboxylase , biology , putrescine , in vitro , polyamine , eflornithine , enzyme inhibitor , biochemistry , enzyme , microbiology and biotechnology
The effects of α‐difluoromethylornithine (DFMO) on in vitro ornithine decarboxylase (ODC) activities from three plant pathogenic fungi, Pyrenophora avenae, Pyricularia oryzae and Uromyces viciae‐fabae , were studied. DFMO concentrations from 0·01 to 1·0 mmol/l produced no significant effects on ODC activities from the three fungi. However, increasing the DFMO concentration to 5 mmol/l produced a substantial reduction in in vitro ODC activity from Pyre, avenae. The ODC inhibitor, α‐monofluoromethylornithine (2 mmol/l), significantly reduced in vitro ODC activity from Pyre. avenae , whereas RR‐methyl acetylenic putrescine, an ODC inhibitor based on putrescine, produced no significant effect on the fungal enzyme.