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A new method for the quantitative determination of microbial extracellular polysaccharide production using a disposable ultrafilter membrane unit
Author(s) -
Toba T.,
Uemura H.,
Itoh T.
Publication year - 1992
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1992.tb00640.x
Subject(s) - polysaccharide , extracellular polysaccharide , lactobacillus , lactobacillaceae , chromatography , microorganism , membrane , extracellular , food science , chemistry , colorimetry , lactobacillus delbrueckii subsp. bulgaricus , bacteria , biology , microbiology and biotechnology , biochemistry , fermentation , genetics
A new method was developed for the determination of extracellular polysaccharides produced by microorganisms. Culture supernatants were ultrafiltered through a membrane having a molecular exclusion limit of 5000 Da. Mono‐ and disaccharides were removed by repeated washings with water and then the polysaccharide in the retentate was determined by colorimetry. The method has been successfully applied to determine polysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2483 in milk.