Premium
A rapid method for enumerating Salmonella in milk powders
Author(s) -
Fleet G.H.,
Karalis T.,
Hawa A.,
Lukondeh T.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00622.x
Subject(s) - salmonella , agar , xylose , enumeration , food science , microbiology and biotechnology , serotype , lactose , chemistry , tryptic soy broth , bacteria , trypsin , inoculation , enterobacteriaceae , chromatography , biology , fermentation , escherichia coli , biochemistry , mathematics , genetics , combinatorics , gene , immunology , enzyme
A one‐day procedure for enumerating Salmonella in milk powder is described. Milk powder suspension was treated with a mixture of trypsin and Tween 80, then centrifuged to give a sediment of microbial cells that was plated on the xylose lysine desoxycholate agar for the direct enumeration of Salmonella. Known populations (1–200 cfu/25 g of sample) of salmonellas inoculated into milk powder suspensions were recovered with almost 100% efficiency in each of 152 trials of the method, covering 17 different serotypes.