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Confocal microscopy of surface‐ and cytoplasmically‐labelled bacteria immobilized by APS‐centrifugation
Author(s) -
Barer M.R.,
Entwistle A.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00604.x
Subject(s) - centrifugation , fluorescein , confocal microscopy , confocal , fluorescein isothiocyanate , propidium iodide , bacteria , microscopy , fluorescence microscope , escherichia coli , chemistry , cytoplasm , differential centrifugation , chromatography , staining , microbiology and biotechnology , fluorescence , biophysics , biology , biochemistry , pathology , optics , physics , genetics , medicine , apoptosis , programmed cell death , gene
Cells of Escherichia coli and Staphylococcus aureus were immobilized by centrifugation onto glass coverslips treated with 3‐aminopropyltriethoxysilane (APS‐centrifugation), labelled with fluorescein isothiocyanate (FITC), fixed with acetone and counterstained with propidium iodine (PI). Confocal microscopy of these preparations allowed resolution of the expected surface (FITC) and cytoplasmic (PI) labels and serial optical sections through cells were readily obtained. The APS‐centrifugation technique provides immobilized bacteria in a form which can take full advantage of confocal microscopy.