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Spectrophotometric assay of yeast sterols using a polyene antibiotic
Author(s) -
Rowe S.M.,
Simpson W.J.,
Hammond J.R.M.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00603.x
Subject(s) - polyene , sterol , yeast , filipin , fermentation , chemistry , antibiotics , biochemistry , ergosterol , brewing , microbiology and biotechnology , bacteria , chromatography , biology , cholesterol , genetics
Spectral changes resulting from specific interaction between sterols and the polyene antibiotic filipin have been exploited in a simple sterol assay that is not subject to interference. This can be employed to measure the sterol content of brewing yeast prior to use thus permitting improved control of the fermentation process. It may also be applicable to quantification of fungal biomass.