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Protein A insensitive ELISA detection of staphylococcal enterotoxin B
Author(s) -
Rogemond V.,
Castro J.M. Da Costa,
Delaunay T.,
Cazaux C.,
Guinet R.M.F.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00601.x
Subject(s) - enterotoxin , isotype , monoclonal antibody , biotinylation , antibody , microbiology and biotechnology , protein a , chemistry , conjugate , biology , escherichia coli , immunology , biochemistry , mathematical analysis , mathematics , gene
A sandwich enzyme‐linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat monoclonal antibodies of the IgG2a isotype which were insensitive to protein A even at concentrations greater than 1000 ng/ml.