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The chick embryo test agrees with the mouse bio‐assay for assessment of the pathogenicity of Listeria species
Author(s) -
Notermans S.,
Dufrenne J.,
Chakraborty T.,
Steinmeyer S.,
Terplan G.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00597.x
Subject(s) - listeria , spleen , listeria monocytogenes , biology , pathogenicity , embryo , microbiology and biotechnology , bacteria , immunology , genetics
The pathogenicity of 20 strains of Listeria was determined with the mouse intravenous bio‐assay and the 10‐day chick embryo chorioallontoic membrane test. In the former, survival in the spleen was measured and in the latter, the LD 100 and LD 50 . There was good correlation between the results of the two tests. Listeria monocytogenes strains that grow in the mouse spleen had an LD 100 of < 125 organisms, while strains in which the numbers of organisms in the mouse spleen remain constant had an LD 100 > 125 organisms. Listeria seeligeri, L. innocua, L. welshimeri, L. grayi and L. murrayi did not persist in the spleen and the numbers of organisms used did not kill chick embryos.