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DNA recovery and direct detection of Tn5 sequences from soil
Author(s) -
Selenska Sonja,
Klingmüller W.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00559.x
Subject(s) - ecori , biology , dna , bacteria , dna extraction , genome , polymerase chain reaction , microbiology and biotechnology , genetics , plasmid , gene
Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in Eco RI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.