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Dried gel hybridization in place of Southern hybridization for detection of Listeria monocytogenes DNA fragments
Author(s) -
Wang RongFu,
Cao WeiWen,
Johnson M.G.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00545.x
Subject(s) - agarose , listeria monocytogenes , dna , microbiology and biotechnology , hybridization probe , dna–dna hybridization , agarose gel electrophoresis , listeria , biology , nucleic acid thermodynamics , gel electrophoresis , chemistry , bacteria , biochemistry , genetics , base sequence
A simple, sensitive, dried gel DNA hybridization method for detection of Listeria monocytogenes DNA fragments is described. DNA samples were fractionated on an agarose gel. The gel was then denatured in NaOH‐NaCl and neutralized in Tris‐NaCl. The resulting agarose gel was dried and hybridized with 32 P‐labelled DNA probe. No transfer to nitrocellullose membranes was used.