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Evaluation of matrices in the rumen simulation technique (RUSITEC) for the maintenance of ciliate protozoa
Author(s) -
Hillman K.,
Williams A.G.,
Lloyd D.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00522.x
Subject(s) - rumen , protozoa , hay , biology , matrix (chemical analysis) , inert , food science , microbiology and biotechnology , zoology , fermentation , chemistry , chromatography , organic chemistry
A number of inert matrices have been evaluated with a rumen simulation technique (RUSITEC) to provide a more defined culture medium for the cultivation of rumen protozoa in vitro . All of the inert matrices tested retained rumen protozoa longer than vessels run without a matrix (up to 7 d with matrix, less than 3 d with no matrix). A semi‐inert digested hay matrix retained protozoa for up to 13 d, as did fresh hay.