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Protein export during growth of Bacillus subtilis : the effect of extracellular protease deficiency
Author(s) -
Coxon R.D.,
Harwood C.R.,
Archibald A.R.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00513.x
Subject(s) - newcastle upon tyne , bacillus subtilis , microbiology and biotechnology , protease , medical school , library science , art , medicine , chemistry , biology , art history , computer science , bacteria , biochemistry , enzyme , medical education , genetics
Exponential phase cultures of a protease‐deficient and a protease‐proficient strain of Bacillus subtilis , growing in minimal medium with a glucose carbon source, were labelled with 14 C‐phenylalanine. Subsequent export of labelled protein was detected in trichloroacetic acid‐precipitated supernatant fluid samples. During exponential growth the strains exhibited substantially similar protein export kinetics. However, as confirmed by the presence of a cytoplasmic enzyme in the culture supernatant fluid, protease deficiency was associated with an increased tendency of cells to lyse as they approached stationary phase.