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A 6 h microslide immunodiffusion assay for confirmed detection of staphylococcal enterotoxins
Author(s) -
McLandsborough L.,
Tatini S.R.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00510.x
Subject(s) - enterotoxin , immunodiffusion , microbiology and biotechnology , staphylococcus aureus , radial immunodiffusion , staphylococcus , chemistry , incubation , micrococcaceae , biology , chromatography , bacteria , antigen , antibody , antibacterial agent , antibiotics , escherichia coli , immunology , biochemistry , genetics , gene
Use of appropriately balanced immunological reagents and incubation of microslides at 45°C resulted in confirmed assay results in 6 h for staphylococcal enterotoxins. The sensitivity of the 45°C–6 h assay was comparable to the 37°C‐24 h assay: 0·1 μg of staphylococcal enterotoxin A/ml.