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Diaminopimelic acid uptake by Bacillus megaterium: influence of growth conditions and other amino acids
Author(s) -
Gally D.,
Harwood C.R.,
Archibald A.R.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00502.x
Subject(s) - newcastle upon tyne , bacillus megaterium , medical school , microbiology and biotechnology , library science , medicine , biology , history , bacteria , art history , computer science , medical education , genetics
Diaminopimelic acid (DAP) uptake was studied in Bacillus megaterium. The K m and V max were determined for bacteria grown with or without DAP. The uptake of DAP was shown to be constitutive and unaffected by the presence of other amino acids (including cystine). The concentration of DAP and lysine in the amino acid pool was examined and a procedure for pulse‐labelling cell walls developed.