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Generation of deletions in pTV1 ts following transformation of Staphylococcus aureas protoplasts
Author(s) -
Luchansky J.B.,
Faith Nancy G.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00500.x
Subject(s) - protoplast , plasmid , transformation (genetics) , biology , staphylococcus aureus , chloramphenicol , erythromycin , microbiology and biotechnology , dna , staphylococcus , antibiotics , bacteria , genetics , gene
Plasmid pTV1 ts was introduced into Staphylococcus aureus by transformation of protoplasts at frequencies ranging from 6·6 times 10 5 to 2·8 times 10 5 per μg of DNA when selection was made for resistance to chloramphenicol and erythromycin, respectively. Phenotypic analysis of regenerated transformants demonstrated three distinct classes. Analysis of the plasmid profiles of several isolates revealed that two classes harboured deleted pTV1 ts derivatives.