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Electroporation‐induced transformation of Escherichia coli: evaluation of a square waveform pulse
Author(s) -
Elvin S.,
Bingham A.H.A.
Publication year - 1991
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1991.tb00498.x
Subject(s) - electroporation , transformation (genetics) , waveform , escherichia coli , pulse (music) , plasmid , square (algebra) , enterobacteriaceae , strain (injury) , microbiology and biotechnology , voltage , biology , physics , mathematics , genetics , anatomy , gene , geometry , quantum mechanics
Four strains of Escherichia coli were tested for electroporation‐induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a sinusoidal waveform of exponential decaying pulse.