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Production of β‐glucosidase (cellobiase) by Curvularia sp.
Author(s) -
Banerjee U. C.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb01332.x
Subject(s) - enzyme , divalent , yeast extract , enzyme assay , chemistry , extracellular , curvularia , cellulase , yeast , chromatography , biochemistry , biology , food science , fermentation , organic chemistry , penicillium
A Curvularia sp. isolated from soil was found to produce extracellular β‐glucosidase activity when grown in yeast extract, peptone, carboxymethylcellulose (YPC) medium. An initial medium pH of 6·5 and cultivation temperature of 30°C were found to be most suitable for high enzyme productivity. The pH and temperature optima for the enzyme were 4·0 and 70°C, respectively. Under these conditions, the enzyme exhibited a K m (0‐nitrophenyl‐β‐ d ‐glucoside) value of 0.20 mmol/l. Several divalent metal ions inhibited enzyme activity at high concentration. EDTA. also inhibited β‐glucosidase activity.