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Improvements to a lysine medium for detection of salmonellas by elctrical conductance
Author(s) -
Smith P. J.,
Bolton F. J.,
Gayner V. E.,
Eccles A.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb01281.x
Subject(s) - conductance , salmonella , lysine , sodium , microbiology and biotechnology , enterobacteriaceae , chemistry , toxicity , bacteria , food science , biology , biochemistry , escherichia coli , amino acid , genetics , mathematics , organic chemistry , combinatorics , gene
Improvements in performance of a lysine conductance medium for the detection of salmonellas were achieved from a study of the effects of its various components. When sodium biselenite was included as an inhibitor for non‐salmonella organisms conductance signals were depressed. The inclusion of sodium chloride reduced this toxicity and improved conductance responses. Increasing the pH to pH 7.0 prevented the medium becoming too acidic and inhibitory to salmonellas. The new medium detected 70‐0% of salmonella‐positive animal protein samples.