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Genetic transformation of intact cells of Lactobacillus curvatus Lc2‐c and Lact. sake Ls2 by elctroporation
Author(s) -
Gaier W.,
Vogel R. F.,
Hammes W. P.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb01280.x
Subject(s) - electroporation , starter , transformation (genetics) , plasmid , lactobacillus , lactobacillaceae , food science , biology , bacteria , fermentation starter , microbiology and biotechnology , chemistry , biochemistry , lactic acid , fermentation , genetics , dna , gene
An electroporation‐induced transformation system was developed for two raw sausage starter organisms: Lactobacillus curvatus Lc2‐c and Lact. sake Ls2. Several plasmids of different origin (pAMβ1, pIL253, pNZ12 and pLP825) were tested for transformation and expression of their resistance determinants. Transfer rates were strongly dependent on growth phase and voltage. Several parameters were studied with possible influence on trasformation efficiencies.