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pIF21, a versatile donor of transposons Tn 1 , Tn 5 and Tn 7 for tagging and mobilizing cryptic and non‐selectable plasmids
Author(s) -
Feavers I.M.,
Fife A. J.,
Harwood C.R.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00170.x
Subject(s) - plasmid , transposable element , biology , selectable marker , genetics , dna , gene , genome
A plasmid, pIF21, has been constructed that is able to donate transposons Tn 1 , Tn 5 and Tn 7 . The transposons are located on a temperature‐sensitive derivative of the inc P1 plasmid pRP1, which is transferable to a wide range of Gram‐negative genera.