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Cloning and expression of alpha‐amylase from Bacillus amyloliquefaciens in a stable plasmid vector in Lactobacillus plantarum
Author(s) -
Jones S.,
Warner P. J.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00164.x
Subject(s) - bacillus amyloliquefaciens , plasmid , biology , microbiology and biotechnology , shuttle vector , expression vector , lactobacillus plantarum , cloning (programming) , vector (molecular biology) , bacteria , gene , genetics , recombinant dna , computer science , lactic acid , programming language
Lactobacillus plantarum is used in a wide range of agricultural and food fermentations. In this paper we report the introduction of alpha‐amylase into the organism from Bacillus arnyloliquefaciens on a stable recombinant plasmid. The genetically manipulated organism grew on MRSB medium supplemented with starch and it may be a prototype for the development of lactobacilli able to use an increased range of substrates in commercial fermentations