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The use of tissue culture for the detection of mycotoxins
Author(s) -
Robb Jean,
Norval Mary,
Neill W. A.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00105.x
Subject(s) - mycotoxin , cytotoxicity , aflatoxin , flow cytometry , biology , incubation , microbiology and biotechnology , food science , biochemistry , in vitro
After incubation with 20 different mycotoxin standards and extracts from fungi and feed stuffs, fluorescent flow cytometry was used to measure viability of NS.1 cells and compared to microscopic assessment of cytotoxicity on stained HEp‐11 monolayers. Both methods gave essentially the same results but the cytometric analysis offered a more quantitative approach and was particularly appropriate in the screening of extracts containing fats. The potential uses of a cytotoxic test in the analysis of feed stuffs and fungal extracts for the presence of mycotoxins are discussed.