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DNA fingerprinting of the mosquito pathogen Bacillus sphaericus with M13 DNA as a probe
Author(s) -
Abadjieva A. N.,
Grigorova R. T.,
Miteva V. I.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00101.x
Subject(s) - bacillus sphaericus , biology , dna , dna profiling , hypervariable region , dna–dna hybridization , microbiology and biotechnology , nucleic acid thermodynamics , hybridization probe , genetics , pathogen , bacillales , virology , bacteria , gene , base sequence , bacillus subtilis
Hypervariable nucleotide sequences were detected in Bacillus sphaericus by hybridization with radioactively labelled M13 DNA. Different serotypes could be distinguished by their hybridization profiles. The appearance of bands common for mosquito‐pathogenic strains and their absence in an apathogenic strain opens the probability that M13 could hybridize to specific alleles, related to insect toxicity.