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Comparison between Rhizobium galegae and Rhizobium meliloti plasmid contents
Author(s) -
SelenskaTrajkowa S.,
Radewa G.,
Markov K.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00097.x
Subject(s) - rhizobium , plasmid , biology , rhizobiaceae , microbiology and biotechnology , botany , genetics , symbiosis , bacteria , dna
Plasmid profiles of two strains of a newly classified rhizobial species‐ Rhizobium galegae ‐were compared with the profiles of several strains of another fast‐growing Rhizobium species‐ Rhizobium meliloti . The existence of a plasmid DNA band with a lower electrophoretic mobility than the R. meliloti megaplasmid band was demonstrated in the two R. galegae strains by a modified horizontal Eckhardt method. Thus R. galegae species contain giant plasmid(s) larger than the R. meliloti 1000 MD megaplasmids, previously considered to be the largest plasmids in the Rhizobiaceae family. In one of the R. galegae strains an additional middle‐size plasmid only a little smaller than 140 MD pRme41a of R. meliloti 41 was observed.