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Antibiotic sensitivity testing of bacteria by microcolony inhibition and image analysis
Author(s) -
Hammonds S. J.,
Adenwala F.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00087.x
Subject(s) - clinical microbiology , library science , antibiotic sensitivity , antibiotics , medicine , microbiology and biotechnology , biology , computer science
A rapid method for determining the sensitivity of Staphylococcus aureus to β‐lactarns is described. The method involves the culture of microcolonies of the bacterium on the surface of antibiotic‐containing agar medium on microscope slides. The areas of the microcolonies formed are measured by means of a microscope and image analyzer. The sensitivity of the bacterium can be estimated in 4 h by this technique and the results are comparable to those obtained by overnight incubation.