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Separation of micro‐organisms from meat and their rapid enumeration using a membrane filtration‐epifluorescent microscopy technique
Author(s) -
Walls Isabel,
Sheridean J. J.,
Welch R. W.,
McDowell D. A.
Publication year - 1990
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1990.tb00086.x
Subject(s) - enumeration , filtration (mathematics) , biology , microbiology and biotechnology , chromatography , bacteria , microscopy , membrane , chemistry , mathematics , physics , biochemistry , optics , statistics , genetics , combinatorics
A new method of separating bacteria from beef mince has been developed, in which an alkaline protease, Alcalase 0.6 L, was used to degrade the meat proteins, leaving micro‐organisms in suspension. The organisms were then counted, using a membrane filtration‐epifluorescent microscopy technique. A correlation coefficient of 0.9? was obtained between this method and the standard plate count, indicating its suitability for use in quality control.