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Rapid enumeration of viable micro‐organisms by staining and direct microscopy
Author(s) -
Betts R.P.,
Bankes P.,
Banks J.G.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00324.x
Subject(s) - enumeration , tetrazolium chloride , formazan , staining , stain , bacteria , membrane filter , microbiology and biotechnology , strain (injury) , biology , plate count , chemistry , membrane , biochemistry , mathematics , cardiology , medicine , genetics , ischemia , anatomy , combinatorics
A rapid method is described that uses the tetrazolium salt 2‐( p ‐iodophenyl‐3‐( p ‐nitrophenyl)‐5‐phenyl tetrazolium chloride (INT) to stain viable micro‐organisms retained on a filter membrane. Good correlation exists between numbers of INT stained cells and aerobic plate counts of single strain cultures of bacteria and yeasts. A pre‐treatment step allows the technique to be used for pasteurized milk.