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Sphaeroplast and electroporation‐mediated transformation of Lactobacillus plantarum
Author(s) -
Badii R.,
Jones S.,
Warner P. J.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00286.x
Subject(s) - electroporation , organism , transformation (genetics) , lactobacillus plantarum , biology , plasmid , model organism , microbiology and biotechnology , lactobacillaceae , lactobacillus , strain (injury) , bacteria , computational biology , genetics , dna , gene , lactic acid , anatomy
Lactobacillus plantarum is an organism of great potential in food and agricultural fermentations. This paper describes two techniques for transformation of the organism: firstly, sphaeroplast transformation and secondly, electroporation‐mediated transformation. Both methods appear to be plasmid‐ and strain‐specific and therefore may be complementary in the development of genetic manipulation of this organism.