Premium
Rapid extraction of bacterial genomic DNA with guanidium thiocyanate
Author(s) -
Pitcher D.G.,
Saunders N.A.,
Owen R.J.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00262.x
Subject(s) - genomic dna , biology , dna , bacteria , microbiology and biotechnology , lysis , escherichia coli , nuclease , biochemistry , genetics , gene
A method is described for the rapid isolation and purification of bacterial genomic DNA. A total of 215 bacterial strains representing species of Campylobacter, Corynebacterium, Escherichia, Legionella, Neisseria, Staphylococcus and Streptococcus , were lysed with guanidium thiocyanate. DNA was prepared using just three other reagents and one high‐speed centrifugation step. The method, which was applicable to both Gram‐positive and Gram‐negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments. The DNA was of high purity, high molecular mass and double‐stranded.