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Enhancement by cationic compounds of the growth inhibitory effect of novobiocin on Escherichia coli
Author(s) -
Modha Jayshree,
BarrettBee K.J.,
Rowbury R.J.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00252.x
Subject(s) - novobiocin , escherichia coli , putrescine , guanidine , chemistry , biochemistry , spermidine , biology , enzyme , gene , antibiotics
The inhibitory effect of low levels of novobiocin on the growth of Escherichia coli was greatly enhanced by the presence of cationic compounds which alone produced no inhibitory effect. Diaminoacetone and spermidine were the most effective enhancers of the novobiocin effect, but N‐(2‐pyrimidinyl)‐piperazine, methylglyoxyl‐bis‐(guanylhydrazone), agmatine, 3,3‐diamino benzidine and 3‐amino benzamidine also produced marked enhancement. There was slightly less effect of 4,5,6‐triamino pyrimidine, pyridoxamine and 1,4‐diamino piperazine whilst 1,3‐diamino guanidine, moroxydine, tetra methyl p ‐phenylene diamine and 1,4‐diamino‐2‐butanone were essentially ineffective as enhancers of novobiocin inhibition. It is suggested that the effective agents aid the penetration of novobiocin through the outer membrane.