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Inducibility and stability of auxotrophic mutations in Mycobacterium fortuitum, M. smegmatis and M. vaccae
Author(s) -
Subramanyam V.R.,
Pal B.B.,
Mohanty Keshari K.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00238.x
Subject(s) - mycobacterium smegmatis , mycobacterium fortuitum , mutant , auxotrophy , microbiology and biotechnology , biology , bacteria , mycobacterium , genetics , gene , medicine , mycobacterium tuberculosis , tuberculosis , pathology
Stable auxotrophic mutants were isolated by N‐methyl‐N'‐nitro‐N‐nitrosoguanidine treatment of Mycobacterium fortuitum NIHJ 1615, M. smegmatis NIHJ 1628 and M. vaccae NIHJ 1637. The number of stable mutants obtained were 0.17, 0.46 and 0.02% of the surviving mutagenized cells screened for the three species, respectively. Mutants differed from their parents in a single nutritional requirement except in the case of SM29, a mutant of M. fortuitum , and SM33, a mutant of M. smegmatis , which differed from their parents in two auxotrophic traits.