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d ‐Galacturonandigalacturonohydrolase covalently bound on to a polyacrylamide‐type support
Author(s) -
Heinrichova K.,
Dzúrova M.,
Zioêki A.,
Wojciechowicz M.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00234.x
Subject(s) - polyacrylamide , covalent bond , carbodiimide , polyacrylamide gel electrophoresis , chemistry , polymer chemistry , enzyme , chromatography , biochemistry , organic chemistry
d ‐Galacturonandigalacturonohydrolase was immobilized by covalent coupling on to a polyacrylamide‐type carrier BIO Gel CM100, activated by water‐soluble carbodiimide. Catalytic properties, stability and action pattern of the immobilized enzyme are reported.