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An alternative approach to the yeast extract‐nalidixic acid method for determining the proportion of metabolically active aquatic bacteria
Author(s) -
AlHadithi S.A.,
Goulder R.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00229.x
Subject(s) - nalidixic acid , bacteria , yeast , biology , microbiology and biotechnology , biochemistry , antibiotic resistance , genetics
When metabolizing aquatic bacteria were determined by the conventional approach to the yeast extract‐nalidixic acid method the apparent percentage of viable bacteria depended on the critical length beyond which cells were scored as viable. An alternative approach allowed the identification of an optimum critical length and the fixing of a lower limit to the true percentage of viable bacteria.