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A medium designed for monitoring pitching yeast contamination in beer using a conductimetric technique
Author(s) -
Adams M.R.,
Bryan J.J.,
Thurston† P.J.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00222.x
Subject(s) - contamination , yeast , conductometry , environmental science , chemistry , chromatography , environmental chemistry , microbiology and biotechnology , biology , ecology , biochemistry
Nitrogen assimilation is the most readily utilized source of conductance changes when pitching yeast is grown in a glucose‐based medium. A simple growth medium comprising yeast nitrogen base, in which nitrogen is supplied as ammonium sulphate, and glucose gave good growth but little change in conductance. Inclusion of a succinate buffer in the medium to remove protons liberated as a result of nitrogen uptake produced a large decrease in conductance and detection times that correlated well with enumeration of yeast by plate counting. The medium will allow more rapid and automated detection of pitching yeast survival in pasteurized beer although individual calibration for each beer type will be necessary.