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Instability of actinomadurae protoplasts generated by lytic enzyme treatment
Author(s) -
Allison D.G.,
Roberts P.M.
Publication year - 1989
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1989.tb00210.x
Subject(s) - lytic cycle , library science , microbiology and biotechnology , biology , virology , virus , computer science
The effect of lytic enzyme treatment upon protoplast formation and reversion in three species of Actinomadura has been determined. Incubation in the presence of lytic enzyme L2 generates large protoplasts (4 μm diameter) which remain intact for only 2h. In comparison, protoplasts formed by the degradative effects of the enzymes lysozyme and L1 are smaller (2 μm diameter), and remain stable for up to 18h. This results in a greater efficiency of regeneration. Lytic enzyme L2 has been shown to contain impurities, including proteolytic activity, which may affect cell wall regeneration.