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Subcloning of β ‐glucanase genes from Ruminococcus albus, Clostridium thermocellum and Butyrivibrio fibrisolvens using the shuttle vector pSA3
Author(s) -
Mann S.P.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01306.x
Subject(s) - clostridium thermocellum , subcloning , ruminococcus , shuttle vector , biology , microbiology and biotechnology , gene , genetics , cellulase , vector (molecular biology) , bacteria , recombinant dna , biochemistry , cellulose
Genes expressing β ‐glucanase activity have been subcloned using the shuttle vector pSA3. Expression has been obtained using Clostridium thermocellum, Ruminococcus albus , and Butyrivibrio fibrisolvens genes in Enterococcus facaelis and by the C. thermocellum gene in Bacillus subtilis . Some subclones proved lethal when β ‐glucanase activity was expressed in conjunction with tetracycline resistance in Escherichia coli .