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Diffusion gradient plates for herbicide toxicity tests on micro‐algae and cyanobacteria
Author(s) -
Paterson D.M.,
Wright S.J.L.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01259.x
Subject(s) - cyanobacteria , algae , atrazine , toxicity , biology , environmental chemistry , botany , chemistry , pesticide , bacteria , ecology , organic chemistry , genetics
Single dimension agar gel diffusion gradient plates were used to test the tolerance of several micro‐algae and cyanobacteria to five herbicides. Growth of most of the test organisms was prevented by terbutryn and diuron at concentrations between 1·1 and 2·8 μg/ml (minimum inhibitory concentration, MIC). Chlorpropham was intermediate in toxicity whilst monuron and atrazine generally gave higher MIC values (up to 17·1 μg/ml). The cyanobacteria were mostly more tolerant than the algae, but susceptibility to the herbicides was variable even between strains of an individual algal species. The gradient plate technique offers the advantages of simplicity and low cost in toxicity screening studies with herbicides or other biologically active chemicals and xenobiotics.