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Production of a monoclonal antibody specific for Salmonella spp.
Author(s) -
Paterson G.E.,
Tiffin A.I.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01248.x
Subject(s) - monoclonal antibody , salmonella , isotype , microbiology and biotechnology , bacteria , biology , enterobacteriaceae , lipopolysaccharide , antibody , escherichia coli , immunology , biochemistry , gene , genetics
A monoclonal antibody (MAb) specific for heat‐treated Salmonella spp. which shows no cross‐reactivity with other enteric bacteria when tested by an ELISA system, has been developed. The MAb isotype is IgG2a and has been successfully purified using protein A. Preliminary work suggests that the MAb recognizes a protein present in the Boivin lipopolysaccharide extracts of Salmonella spp.