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Studies on pre‐incubation conditions in the seeded agar plate well inhibition assay
Author(s) -
Cannell R.J.P.,
Knowles M.,
Walker J.M.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01242.x
Subject(s) - incubation , agar , incubation period , agar plate , seeding , microbiology and biotechnology , algae , chemistry , chromatography , bacterial growth , agar diffusion test , biology , bacteria , botany , biochemistry , antibacterial activity , genetics , agronomy
Pre‐incubation at 4°C increases the zone of bacterial growth inhibition produced by methanolic extracts of freshwater algae in the agar diffusion assay. Preloading of wells with methanol increases the inhibition zone produced by water‐insoluble antibiotics.