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Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F 1 ‐ATPase preparations by polyacryacrylamide gel electrophoresis
Author(s) -
Niskasaari Kaarina,
Juutinen Katri M.S.,
Rimpiläinen Marja A.,
Forsén Raili
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01238.x
Subject(s) - atpase , lipoteichoic acid , staining , biochemistry , membrane , polyacrylamide gel electrophoresis , gel electrophoresis , molecular mass , adenosine triphosphatase , antiserum , biology , chromatography , triphosphatase , chemistry , bacteria , enzyme , antibody , genetics , immunology , staphylococcus aureus
The presence of lipoteichoic acid (LTA) in plasma membrane and released adenosine triphosphatase (ATPase) preparations of Streptococcus lactis subsp. cremoris HA was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). The membrane preparation gave two spots with periodic acid Schiff's (PAS) staining. The reference LTA revealed one CBB stainable band with MW 21 500, and was detected as a characteristic yellow spot in the molecular weight area 14 000–20 900 with silver staining and was also stainable with PAS. A slight colour was found with PAS in the F 1 ‐ATPase (EC 3.6.1.3) preparation. The results suggest that the carbohydrate component in the membrane preparation and in the solubilized F 1 ‐ATPase is LTA.