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N,N ‐Diacetylchitobiose production from chitin by Vibrio anguillarum strain E‐383a
Author(s) -
Takiguchi Y.,
Shimahara K.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01231.x
Subject(s) - chitin , vibrio anguillarum , microbiology and biotechnology , strain (injury) , library science , biology , vibrio , bacteria , computer science , biochemistry , genetics , anatomy , chitosan
Vibrio anguillarum strain E‐383a, isolated from sea water, accumulated a considerable amount of N,N ‘‐diacetylchitobiose when it was cultivated in a medium containing colloidal chitin. The maximum conversion of chitin to chitobiose was found to be 40·3%. The rate of chitobiose accumulation was accelerated after the cessation of bacterial growth. Small amounts of N ‐acetylglucosamine and N,N’,N ‐triacetylchitotriose were also accumulated but no other saccharides were detected. These results may suggest that strain E‐383a produces an exo‐type chitinase which successively hydrolyses the glycosidic linkages of chitin into biose units. The exclusive accumulation of chitobiose by the bacterial cells may provide a new, selective method for the production of this substance.