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Evaluation of the stain Viablue for the rapid estimation of viable yeast cells
Author(s) -
Hutcheson T.C.,
McKay T.,
Farr L.,
Seddon B.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01220.x
Subject(s) - differential staining , staining , yeast , stain , biology , fluorescence microscope , fluorescent staining , filter paper , microbiology and biotechnology , fluorescence , chemistry , chromatography , biochemistry , genetics , optics , physics
Viablue can be used as a differential viability stain for yeast cells. Differential staining using fluorescence microscopy and viability showed a good correlation ( r = 0·99). For practical purposes the staining procedure takes only a few minutes and appears ideally suited to such rapid analytical procedures as the direct epifluorescent filter technique (DEFT) and automated quality control testing of foods and beverages.