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Molecular analysis of an aesculinase activity from Klebsiella oxytoca
Author(s) -
ALZAAG A.,
Pemberton J. M.
Publication year - 1988
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1988.tb01203.x
Subject(s) - klebsiella oxytoca , plasmid , pbr322 , escherichia coli , biology , aeromonas hydrophila , microbiology and biotechnology , klebsiella , enterobacteriaceae , recombinant dna , molecular cloning , gene , salmonella , bacteria , genetics , gene expression
A DNA fragment containing a Klebsiella oxytoca gene for aesculinase activity was cloned on the multicopy plasmid pBR322. This β‐glucosidase activity was confined to aesculin hydrolysis only. It was expressed equally well in Escherichia coli, Salmonella typhimurium and Aeromonas hydrophila. Two polypeptides were found to be encoded within the 2·6 kb of the cloned DNA encoding aesculinase activity.