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Adsorption of Thermomonospora curvata cellulases on insoluble substrates
Author(s) -
WILLIAMSON B. N.,
STUTZENBERGER F. J.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01624.x
Subject(s) - cellulase , adsorption , filter paper , chemistry , cellulose , cellulosic ethanol , enzyme , chromatography , langmuir adsorption model , saturation (graph theory) , biochemistry , organic chemistry , mathematics , combinatorics
Cellulosic substrates were tested for their ability to bind the cellulases of Thermomonospora curvata . Protein‐extracted lucerne fibres had the highest adsorptive capacity while native cotton fibres had the least. Analysis of binding as a Langmuir adsorption isotherm yielded maximal binding capacities of 0.012 filter paper units and 0.51 endoglucanase units/mg lucerne fibre at enzyme saturation. This capacity was increased about 12‐fold by fibre pre‐treatment in hot NaOH solution.